Microbial Community Profiling:
We offer three for profiling microbial communities:
- Standard 16S - Our standard 16S protocol (Kozich et al., 2013) targets and amplifies the V4 region of bacterial and archaeal 16S rRNA-encoding genes.
- 16S EMP - A modified set of primers by the Earth Microbiome Project (EMP) that also targets the V4 region but with added degeneracies to be more inclusive of bacterial and archaeal species found in some environments (Walters et al., 2015).
- Fungal ITS - Primers targeting the fungal Internally Transcribed Spacer (ITS) region for amplification of a broad spectrum of fungal taxa in complex microbial communities (Taylor et al., 2016).
Community profiling can occur on purified DNA samples from a variety of sources, including blood, stool, saliva and soil. We accept samples in a 96-well plate format, with up to 94 samples and 2 blank wells per controls. Purified nucleic acids can be quantified at the Core, or we will process the samples as described in our Nucleic Acid Extraction service. Libraries are cleaned, normalized, and undergo quality control before sequencing on the Illumina MiSeq platform. Output data (ex: FASTQ files) can be returned to our clients via Illumina’s BaseSpace Sequence Hub or on a USB flash drive.
If you have further questions, please refer to the Frequently Asked Questions page.